分子量:64 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human IL-1 RII, Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:Measured by its binding ability in a functional ELISA.Immobilized Human IL-1 RII, Fc Tag (Cat# IL2-H4256) at 10 μg/mL (100 L/well),can bind Biotinylated Human IL-1 alpha (Cat# ILA-H8213) with a linear range of 0.05-1 μg/mL.

产品特性:Human IL-1 RII, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 64 kDa. The predicted N-terminus is Thr 14. The reducing (R) protein migrates as 68-74 kDa in SDS-PAGE  due to glycosylation.

产品背景:Interleukin-1 receptor type 2 (IL1R2) is also known as CD121 antigen-like family member B (CDw121b), IL-1 type II receptor, Interleukin-1 receptor type II, belongs to the interleukin-1 receptor family. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R2 is non-signaling receptor for IL1A, IL1B and IL1RN, reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. IL1R2 modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.