Recombinant Human Dickkopf-related protein-1,His (Human DKK-1,His )
产品名称： Recombinant Human Dickkopf-related protein-1,His (Human DKK-1,His )
英文名称： Recombinant Human Dickkopf-related protein-1,His (Human DKK-1,His )
产品编号： 92275ES20
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T13:09:52
使用范围： null
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Product Information
|
Product Name |
Cat# |
Size |
Price（¥） |
|
Recombinant Human Dickkopf-related protein-1,His (Human DKK-1,His ) |
92275ES20 |
20 μg |
2,675 |
|
92275ES60 |
100 μg |
7,985 |
|
|
92275ES80 |
1 mg |
29,655 |
Product Description
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues . Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway. This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 . Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2. Dkk1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling. Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. Other sites of expression include developing neurons, hair follicles and the retina of the eye .
Product Properties
|
Synonyms |
Dickkopf-1;DKK-1;SK Protein; DKK1; dkk1; dkk-1 |
|
Accession |
O94907 |
|
Source |
HEK293 Cells-derived human Dkk-1 protein, Met2-His 266 with His tag at the C-terminus. |
|
Molecular Weight |
Approximately 27.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh DKK1 is approximately 42.9 kDa due to glycosylation. |
|
AA Sequence |
MALGAAGAT RVFVAMVAAA LGGHPLLGVS ATLNSVLNSN AIKNLPPPLG GAAGHPGSAV SAAPGILYPG GNKYQTIDNY QPYPCAEDEE CGTDEYCASP TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI EETITESFGN DHSTLDGYSR RTTLSSKMYH TKGQEGSVCL RSSDCASGLC CARHFWSKIC KPVLKEGQVC TKHRRKGSHG LEIFQRCYCG EGLSCRIQKD HHQASNSSRL HTCQRH |
|
Tag |
His |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 90% by SDS-PAGE. |
|
Biological Activity |
Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 μg/mL in the presence of 10 ng/mL of mouse Wnt3a. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.
HB220415