低氧诱导因子2α(HIF2α)重组蛋白

YB466Ra011
Hypoxia Hypoxia Hypoxia Hypoxia Inducible Inducible Inducible Inducible Factor 2 Alpha (HIF2a) (HIF2a) (HIF2a) (HIF2a)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
低氧诱导因子2α(HIF2α)重组蛋白7th Edition (Revised in May, 2013)
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Cys339~Gln541 (Accession # Q99814),
with N-terminal His-Tag.
Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Nucleus.
Purity: Purity: Purity: Purity: >95%
低氧诱导因子2α(HIF2α)重组蛋白Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 4.4
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 23.6kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
低氧诱导因子2α(HIF2α)重组蛋白Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-CV NYVLSEIEKN DVVFSMDQTE SLFKPHLMAM NSIFDSSGKG
AVSEKSNFLF TKLKEEPEEL AQLAPTPGDA IISLDFGNQN FEESSAYGKA ILPPSQPWAT
ELRSHSTQSE AGSLPAFTVP QAAAPGSTTP SATSSSSSCS TPNSPEDYYT SLDNDLKIEV
IEKLFAMDTE AKDQCSTQTD FNELDLETLA PYIPMDGEDF Q