ATCC® Number: 
TIB-207™     
Price: 
$268.00 

Designations: 
GK1.5 
Depositors: 
 FW Fitch 

Isotype: 
rat IgG2b 



Biosafety Level:
1 
Shipped: 
frozen 

Medium & Serum: 
See Propagation 
Growth Properties:
suspension

Organism:
Rattus norvegicus (B cell); Mus musculus (myeloma) (rat (B cell); mouse (myeloma)) 
Morphology:
lymphoblast



Source:
Organ: spleen
Cell Type: hybridoma: B lymphocyte;

Cellular Products:
immunoglobulin; monoclonal antibody; against mouse helper, inducer T cells (L3T4 antigen, CD4) 

Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 



Related Cell Culture Products 

Comments:
Animals were immunized with the cloned cytotoxic T lymphocyte lines V4 and 243/2.5.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
L3T4 is expressed on mouse helper/inducer T cells and is analogous to the human Leu-3/T4 molecule. [58010] 
The antibody profoundly blocks antigen specific murine class II MHC antigen reactive helper T lymphocyte lines. [58010] 
Tested and found negative for ectromelia virus (mousepox).

Propagation: 
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20.
Atmosphere: air, 95; carbon dioxide (CO2), 5
Temperature: 37.0°C

Subculturing: 
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml.
Interval: Maintain between 1 X 10(5) and 1 x 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days

Preservation: 
Freeze medium: Complete growth medium supplemented with 5 (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase

Related Products:
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020

References:
982: Fitch FW, et al. Evidence implicating L3T4 class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J. Immunol. 131: 2178-2183, 1983. PubMed: 6195255
983: Dialynas DP, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J. Immunol. 131: 2445-2451, 1983. PubMed: 6415170
32309: Garvy BA, Harmsen AG. The role of T cells in infection-driven interstitial pneumonia after bone marrow transplantation in mice. Transplantation 62: 517-525, 1996. PubMed: 8781619
32945: Murray HW, et al. Models of relapse of experimental visceral leishmaniasis. J. Infect. Dis. 173: 1041-1043, 1996. PubMed: 8603949
32947: Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913
32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902
33098: Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838
33116: Murray HW, et al. Multiple host defense defects in failure of C57BL/6 ep/ep (Pale Ear) mice to resolve visceral Leishmania donovani infection. Infect. Immun. 64: 161-166, 1996. PubMed: 8557335
58010: Dialynas DP, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol. Rev. 74: 29-56, 1983. PubMed: 6195085



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