APOBEC3G polyclonal antibody
产品名称： APOBEC3G polyclonal antibody
英文名称： APOBEC3G polyclonal antibody
产品编号： PAB2469
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of APOBEC3G.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human APOBEC3G.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The human immunodeficiency virus type 1 Vif protein reduces intracellular expression and inhibits packaging of APOBEC3G (CEM15), a cellular inhibitor of virus infectivity.
Kao S, Khan MA, Miyagi E, Plishka R, Buckler-White A, Strebel K.J Virol. 2003 Nov;77(21):11398-407.
- 2.
- HIV-1 Vif blocks the antiviral activity of APOBEC3G by impairing both its translation and intracellular stability.
Stopak K, de Noronha C, Yonemoto W, Greene WC.Mol Cell. 2003 Sep;12(3):591-601.
- 3.
- Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts.
Mangeat B, Turelli P, Caron G, Friedli M, Perrin L, Trono D.Nature. 2003 Jul 3;424(6944):99-103. Epub 2003 May 28.
- Applications
- Western Blot (Cell lysate)
- The APOBEC3G polyclonal antibody (Cat # PAB2469) is used in Western blot to detect APOBEC3G in HL-60 cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the APOBEC3G polyclonal antibody (Cat # PAB2469) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 60489
- Protein Accession#:
- AAG14956
- Gene Name:
- APOBEC3G
- Gene Alias:
- ARP9,CEM15,FLJ12740,MDS019,bK150C2.7,dJ494G10.1
- Gene Description:
- apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
- Omim ID:
- 607113
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq
- Other Designations:
- DNA dC->dU editing enzyme,OTTHUMP00000028911,phorbolin-like protein MDS019
- Gene Pathway