Recombinant Human R-Spondin-1 Protein,His (Human RSPO1,His )
产品名称： Recombinant Human R-Spondin-1 Protein,His (Human RSPO1,His )
英文名称： Recombinant Human R-Spondin-1 Protein,His (Human RSPO1,His )
产品编号： 92274ES60
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T13:22:33
使用范围： null
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Product Information
|
Product Name |
Cat# |
Size |
Price（¥） |
|
Recombinant Human R-Spondin-1 Protein,His (Human RSPO1,His ) |
92274ES60 |
100 μg |
5775.00 |
|
92274ES80 |
1 mg |
31425.00 |
Product Description
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3, is a 27 kDa secreted protein that shares ~40% aa identity with three other R-Spondin family members . All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns. In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender- Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids .
Product Properties
|
Synonyms |
R-Spondin 1 ；Roof Plate-specific Spondin 1 |
|
Accession |
Q2MKA7 |
|
Source |
HEK293 Cells-derived human RSPO1 protein, Met1 22-Ala 263 with His tag at the C-terminus. |
|
Molecular Weight |
Approximately 28.2 kDa. As a result of glycosylation, RSPO1 migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions. |
|
AA Sequence |
MRLGLCVVAL VLSWTHLTIS SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA |
|
Tag |
His |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 95% by SDS-PAGE. |
|
Biological Activity |
1. Measured by its ability to induce activation of ß-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 μg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a. 2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind human LIMPII with a linear range of 32-800 ng/ml. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.
HB220413