Tubulin-alpha monoclonal antibody, clone TU-01
产品名称： Tubulin-alpha monoclonal antibody, clone TU-01
英文名称： Tubulin-alpha monoclonal antibody, clone TU-01
产品编号： MAB3826
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against native Tubulin-alpha.
- Immunogen:
- Native purified Tubulin-alpha from porcine brain alpha tubulin (two cycles of polymerization-depolymerization).
- Host:
- Mouse
- Reactivity:
- Pig
- Specificity:
- This antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
- Form:
- Liquid
- Concentration:
- 1 mg/mL
- Isotype:
- IgG1
- Storage Buffer:
- In PBS, pH 7.4 (15 mM sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1-2 mg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 mg/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Dazap2 modulates transcription driven by the Wnt effector TCF-4.
Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V.Nucleic Acids Res. 2009 May;37(9):3007-20. Epub 2009 Mar 20.
- 2.
- Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells.
Kukharskyy V, Sulimenko V, Macurek L, Sulimenko T, Draberova E, Draber P.Exp Cell Res. 2004 Aug 1;298(1):218-28.
- 3.
- Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells.
Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P.Planta. 1997;201(3):349-58.
- Applications
- Western Blot (Cell lysate)
- Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01 (Cat # MAB3826).
IgM heavy chain and IgM light chain (25-30 KDa) indicated.
MW of alpha tubulin is around 50 KDa.
L = lysate. IPr = immunoprecipitate (reducing conditions). IPn = immunoprecipitate (non-reducing conditions).
- Western Blot (Cell lysate)
- Use of alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826) as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).
- Immunofluorescence
- Immunofluorescence staining of HeLa (human cervix carcinoma cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; red). Nucleus is stained with DAPI (blue).
- Immunofluorescence
- Panel A : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green) and VIM monoclonal antibody, clone VI-01 (Cat # MAB3880 ; red). Nucleus is stained with DAPI (blue).
Panel B : Immunofluorescence staining of NIH/3T3 (mouse embryonal fibroblast cell line) using alpha Tubulin monoclonal antibody, clone TU-01 (Cat # MAB3826 ; green). Nucleus is stained with DAPI (blue).