<p><p><p><p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><strong><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">产品特征：</span></strong></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">Izon品牌qEV系列外泌体（Exosomes）分离试剂盒利用Size Exclusion Chromatography(SEC)的原理分离外泌体。主要是利用溶液中颗粒尺寸的不同来吸附比外泌体更小的比如蛋白，脂类等，是它们更缓慢的通过柱子，从而达到分离和纯化的目的。 </span><br> </p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><strong><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">优势：</span></strong></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">1）去除背底杂蛋白和脂类等, 得到高纯度外泌体；</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">2）简单，方便，快速，样品处理 <30 mins；</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">3) 不引入杂质，无损伤；</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">4）成本低，效率高，适合大量样品处理。</span><br> </p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><strong><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">操作步骤:</span></strong></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">需要准备的材料有：0.2um滤器过滤过的PBS 50ml、15ml离心管、5ml离心管、1.5ml离心管，15ml超滤管、2ml超滤管等。</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">1、收集细胞培养液，2000g 10min 去除死细胞，取上清</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">2、10000g 30min 去除细胞残片，取上清</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">3、110000g 70min 超速离心，取沉淀。PBS重悬沉淀，冲洗沉淀。</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">4、110000g 70min 超速离心，去沉淀。</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">5、重复步骤4一次。</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">6、200ul PBS重悬沉淀即可。 </span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"> </p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">本产品的客户包括世界多家著名研究机构，如剑桥大学、哈佛医学 院、波士顿大学、阿姆斯特丹大学，以及国内清华大 学、中科院上海生命院、复旦大学、上海交通大学、浙 江大学等。</span></p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><br> </p><p style="margin: 0px; padding: 0px; outline: 0px; font-family: 'microsoft yahei', 黑体, helvetica, tahoma, arial, sans-serif; border: 0px; line-height: 18px; background-color: rgb(245, 250, 254);"><strong><span style="outline: 0px; line-height: 22px !important; font-size: 14px;">发布者：上海纳洛捷生物科技有限公司<br style="margin: 0px; padding: 0px;">联系电话：021-54291986,18917300586李经理<br style="margin: 0px; padding: 0px;">E-mail：18917300586@163.com</span></strong></p></p></p></p></p>