唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白

YB790Hu011
Recombinant Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白[ PROPERTIES ]
Residues: Gly70~Ser336
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q9NYZ4
Host: E. coli
Subcellular Location: Membrane; Single-pass type
I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 9.2
Predicted Molecular Mass: 59.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8
oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
G DRPYQDAPVA TNNPDREVQA ETQGRFQLLG DIWSNDCSLS IRDARKRDKG
SYFFRLERGS MKWSYKSQLN YKTKQLSVFV TALTHRPDIL ILGTLESGHS RNLTCSVPWA
CKQGTPPMIS WIGASVSSPG PTTARSSVLT LTPKPQDHGT SLTCQVTLPG TGVTTTSTVR
LDVSYPPWNL TMTVFQGDAT ASTALGNGSS LSVLEGQSLR LVCAVNSNPP ARLSWTRGSL
TLCPSRSSNP GLLELPRVHV RDEGEFTCRA QNAQGS
[ REFERENCES ]
1. Varela I., et al. (2011) Nature 469:539-542.
2. Na H.J., et al. (2012) J. Immunol. Methods 383:39-46.
3. Na H.J., et al. (2012) Cytokine 57:169-174.
4. Hudson S.A., et al. (2011) J. Clin. Immunol. 31:1045-1053.