FITC标记的泛素特异性蛋白酶OTB1抗体-抗体-抗体-生物在线
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FITC标记的泛素特异性蛋白酶OTB1抗体

FITC标记的泛素特异性蛋白酶OTB1抗体

商家询价

产品名称： FITC标记的泛素特异性蛋白酶OTB1抗体

英文名称： Anti-OTUB1/FITC

产品编号： HZ-11229R-FITC

产品价格： null

产品产地： 中国/上海

品牌商标： HZbscience

更新时间： 2023-08-17T10:24:20

使用范围： ICC=1:50-200 IF=1:50-200

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 Rabbit Anti-OTUB1/FITC Conjugated antibody

FITC标记的泛素特异性蛋白酶OTB1抗体

 

产品编号 bs-11229R-FITC
英文名称 Anti-OTUB1/FITC
中文名称 FITC标记的泛素特异性蛋白酶OTB1抗体
别    名 Deubiquitinating enzyme OTUB1; hOTU1; OTB1; OTU domain containing ubiquitin aldehyde binding protein 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU1; Otub1; OTUB1_HUMAN; Otubain 1; Otubain-1; Ubiquitin specific processing protease OTUB1; Ubiquitin thioesterase OTUB1; Ubiquitin-specific-processing protease OTUB1.  
规格价格 100ul/2980元 购买        大包装/询价
说 明 书 100ul  
研究领域 细胞生物  免疫学  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Sheep, 
产品应用 ICC=1:50-200 IF=1:50-200  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 73kDa
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human OTUB1
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍 background:
OTUB1 is a 271 amino acid protein that contains one OTU (ovarian tumor) domain and belongs to the OTU family of predicted cysteine proteases. Expressed as two isoforms (one of which is present throughout the body and the other of which is present only in lymphoid tissues), OTUB1 functions as a hydrolase that can remove ubiquitin residues from target proteins, thereby preventing protein degradation and playing an important role in protein turnover. OTUB1 interacts with GRAIL and, via this interaction, plays a role in the regulation and the induction of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to their cognate antigens. Due to its interaction with GRAIL, OTUB1 is an important regulator of immune responses in secondary lymphoid organs.

Function:
Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin. 
Plays a key non-catalytic role in DNA repair regulation by inhibiting activity of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites. Inhibits RNF168 independently of ubiquitin thioesterase activity by binding and inhibiting UBE2N/UBC13, the E2 partner of RNF168, thereby limiting spreading of 'Lys-63'-linked histone H2A and H2AX marks. Inhibition occurs by binding to free ubiquitin: free ubiquitin acts as an allosteric regulator that increases affinity for UBE2N/UBC13 and disrupts interaction with UBE2V1. The OTUB1-UBE2N/UBC13-free ubiquitin complex adopts a configuration that mimics a cleaved 'Lys48'-linked di-ubiquitin chain. 

Subunit:
Isoform 1 and isoform 2 interact with RNF128. Isoform 1 forms a ternary complex with RNF128 and USP8. Isoform 1 interacts with the C-terminal UCH catalytic domain of USP8. Isoform 2 does not associate with USP8. Interacts with FUS, ESR1 and GNB2L1/RACK1. Interacts with UBE2N/UBC13. 

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Isoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells.

Similarity:
Belongs to the peptidase C65 family. Contains 1 OTU domain.

Database links:
UniProtKB/Swiss-Prot: Q96FW1.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
   
   

OTUB1是包含一个OTU（卵巢肿瘤）结构域的271个氨基酸蛋白，属于预测半胱氨酸蛋白酶的OTU家族。表达为两种异构体（其中一种存在于全身，另一种仅存在于淋巴样组织中），OTUB1起到水解酶的作用，可以除去靶蛋白中的泛素残基，从而防止蛋白质降解并在蛋白质中发挥重要作用。人事变更率。OTUB1与GRAIL相互作用，通过这种相互作用，在T细胞无反应性的调节和诱导中起作用，这是当T细胞对它们的同源抗原反应迟钝时发生的现象。由于与TRAIL的相互作用，OTUB1是次级淋巴器官免疫应答的重要调节因子。