嗜中性粒细胞胞浆因子1(NCF1)重组蛋白

YB677Hu014
Recombinant Neutrophil Cytosolic Factor 1 (NCF1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 嗜中性粒细胞胞浆因子1(NCF1)重组蛋白[ PROPERTIES ]
Residues: Met1~Val390
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P14598
Host: E. coli
Subcellular Location: Cytoplasm, cytosol.
Membrane; Peripheral membrane protein;
Cytoplasmic side.
 嗜中性粒细胞胞浆因子1(NCF1)重组蛋白Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 9.2
Predicted Molecular Mass: 48.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
 嗜中性粒细胞胞浆因子1(NCF1)重组蛋白that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKTLKEMFPI
EAGAINPENR IIPHLPAPKW FDGQRAAENR QGTLTEYCGT LMSLPTKISR CPHLLDFFKV
RPDDLKLPTD NQTKKPETYL MPKDGKSTAT DITGPIILQT YRAIANYEKT SGSEMALSTG
DVVEVVEKSE SGWWFCQMKA KRGWIPASFL EPLDSPDETE DPEPNYAGEP YVAIKAYTAV
EGDEVSLLEG EAVEVIHKLL DGWWVIRKDD VTGYFPSMYL QKSGQDVSQA QRQIKRGAPP
RRS SI R NA HS IH Q RS R K R L S QDAY R R NS V R FL Q Q R R R QA R PGP Q S P GS P L
EEERQTQRSK PQPAVPPRPS ADLILNRCSE STKRKLASAV