分子量:44.6 kDa

纯度:>92% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human CD27 Ligand, Fc Tag by the LAL method.

Buffer:50 mM tris, 100 mM glycine, pH7.5

生物活性:Immobilized mouse CD27 at 2 μg/mL ( 100 μl/well ) can bind human CD70 with a linear  range of 62 - 1000 pg/mL.

产品特性:Human CD27 Ligand, Fc Tag is fused with a human IgG1 Fc tag at the N-terminus, and has a calculated MW of 44.6 kDa. The predicted N-terminus is Glu. The reducing (R) protein migrates as 50-160 kDa in SDS-PAGE  due to monomeric, dimeric or trimeric form and different glycosylation.

产品背景:Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.