生长因子受体结合蛋白7(Grb7)重组蛋白

YB470Mu014
Growth Factor Receptor Bound Protein 7 (Grb7)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
生长因子受体结合蛋白7(Grb7)重组蛋白[ PROPERTIES ]
Residues: Met298~Glu489 (Accession # Q03160),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cytoplasm. Cell projection.
Cell junction, focal adhesion. Cell membrane;
Peripheral membrane protein; Cytoplasmic side.
Cytoplasmic granule.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(生长因子受体结合蛋白7(Grb7)重组蛋白determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 9.8
Predicted Molecular Mass: 25.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
生长因子受体结合蛋白7(Grb7)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSG LVPRGSH MASMTGGQQM GRGSEF -MPT DFGFCVKP NK
LRNGHKGLHI FCSEDEQSRT CWLAAFRLFK YGVQLYKNYQ QAQSRHLRLS YLGSPPLRSV
SDNTLVAMDF SGHAGRVIDN PREALSAAME EAQAWRKKTN HRLSLPTTCS GSSLSAAIHR
TQPWFHGRIS REESQRLIGQ QGLVDGVFLV RESQRNPQGF VLSLCHLQKV KHYLILPSE