上海中乔新舟生物科技有限公司是美国Sciencell公司在中国正规一级代理：

         美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。
         ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。

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产品：大鼠晶状体上皮细胞

货号：R6550
 

Description

The cells that make up the mammalian lens consist of two types: lens fiber cells, which form the bulk of the lens, and a monolayer of epithelial cells cover the anterior surface of the fibers. The normal development of the lens of the eye involves the progressive differentiation and maturation of the lens epithelial cells. As these cells migrate from the equatorial region of the lens into the interior of the lens, they produce the transparent crystallins, elongate to form lens fiber cells and loose their nuclei and other organelles [1]. The causes of lens epithelial cells differentiation are not well understood; however, some progress has been made in determining the underlying molecular and cellular processes of lens epithelial cell differentiation. This process can be promoted by growth factors present in the ocular fluids [2]. Some growth factors, such as epidermal growth factor, promote mitosis; others, such as basic fibroblast growth factor, insulin growth factor, and insulin, promote cell migration and differentiation [3].

RLEpiC from ScienCell Research Laboratories are isolated from the human lens. RLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. RLEpiC are negative for mycoplasma, bacteria, yeast and fungi. RLEpiC are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Epithelial Cell Medium-animal (EpiCM-a, Cat. No. 4131) for the culturing of RLEpiC in vitro.

Product Use

RLEpiC-LE are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference
 

[1]. Wagner, L. M. and Takemoto, D. J. (2001) PKCa and PKC? overexpression causes lentoid body formation in the N/N 1003A rabbit lens epithelial cell line. Molecular Vision 7: 138-144.
[2]. Lang, R. A. (1999) Which factors stimulate lens fiber cell differentiation in vivo? Invest Opthalmol Vis Sci 40:3075-8.
[3]. Leenders, W. P., van Genesen, S. T., Schoenmakers, J. G., van Zoelen, E. J., Lubsen, N. H. (1997) Synergism between temporally distinct growth factors: bFGF, insulin and lens cell differentiation. Mech Dev. 67:193-201.