层粘连蛋白β2(LAMβ2)重组蛋白

YB080Ra031
Recombinant Laminin Beta 2 (LAMb2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
层粘连蛋白β2(LAMβ2)重组蛋白[ PROPERTIES ]
Residues: Asp38~His350
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P55268
Host: E. coli
Subcellular Location: Secreted.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
层粘连蛋白β2(LAMβ2)重组蛋白Predicted isoelectric point: 6.6
Predicted Molecular Mass: 65.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
层粘连蛋白β2(LAMβ2)重组蛋白DVP GCSRGSCYPA TGDLLVGRAD RLTASSTCGL NGPQPYCIVS HLQDEKKCFL
CDSRRPFSAR DNPHSHRIQN VVTSFAPQRR AAWWQSENGI PAVTIQLDLE AEFHFTHLIM
TFKTFRPAAM LVERSADFGR TWHVYRYFSY DCGADFPGVP LAPPRHWDDV VCESRYSEIE
PSTEGEVIYR VLDPAIPIPD PYSSRIQNLL KITNLRVNLT RLHTLGDNLL DPRREIREKY
YYALYELVVR GNCFCYGHAS ECAPAPGAPA HAEGMVHGAC ICKHNTRGLN CEQCQDFYRD
LPWRPAEDGH SHACRKCECH