异染色质蛋白1-α单克隆抗体
产品名称： 异染色质蛋白1-α单克隆抗体
英文名称： HP1 alpha
产品编号： hzm-33410M
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ELISA=1:1000-5000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
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Mouse Anti-HP1 alpha antibody
| 产品编号 | hzm-33410M |
| 英文名称 | HP1 alpha |
| 中文名称 | 异染色质蛋白1-α单克隆抗体 |
| 别 名 | Antigen p25; HP1-α; CBX5; CBX5_HUMAN; Chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha. HP-1-α; HP 1α; HP-1α; HP-1 α; HP1α; HP1 α; |
| 研究领域 | 免疫学 转录调节因子 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 4A7 |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | ELISA=1:1000-5000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 22kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HP1 alpha Protein: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin. Function: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Subunit: Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1. Subcellular Location: Nucleus. Chromosome. Chromosome, centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. Post-translational modifications: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. Ubiquitinated. Similarity: Contains 2 chromo domains. SWISS: P45973 Gene ID: 23468 Database links: Entrez Gene: 538885 Cow Entrez Gene: 477593 Dog Entrez Gene: 23468 Human Entrez Gene: 12419 Mouse Entrez Gene: 300266 Rat Omim: 604478 Human SwissProt: P45973 Human SwissProt: Q61686 Mouse Unigene: 349283 Human Unigene: 262059 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic application |