人内吞作用定量PCR芯片试剂盒
产品名称: 人内吞作用定量PCR芯片试剂盒
英文名称: GQH-ECY
产品编号: GK022
产品价格: 0
产品产地: 美国
品牌商标: sciencell
更新时间: 2024-12-16T09:19:24
使用范围: null
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- 地址 : 上海市宝山区长江南路180号A区401-406室
- 邮编 : 200433
- 所在区域 : 上海
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人内吞作用定量PCR芯片试剂盒描述:
ScienCells GeneQuery™ Human Endocytosis qPCR Array Kit (GQH-ECY) is designed to facilitate gene expression profiling of 88 key genes involved in cellular endocytosis. Endocytosis pathways are used to mediate signal transduction from the cell surface and to internalize or transport particulate matter. Brief examples of how included genes may be grouped according to endocytic pathway are shown below:
- Clathrin-mediated: CLTA, CLINT1, AP2A1, SNX9, EPN1
- Caveolae: CAV1, CAV2, CAV3, PTRF, FLOT1
- Macropinocytosis: KRAS, SRC, PAK1, PAK2, HDAC6
- Phagocytosis and degradation: IQGAP1, ROCK1, UBB, DCTN1, AMPH
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Endocytosis qPCR Array Plate Layout* (8 controls in Bold and Italic)
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ACTB |
ARHGAP26 |
CLTB |
DOCK1 |
EPN1 |
IQGAP1 |
MAPK8IP3 |
PIKFYVE |
RAB11FIP5 |
RALBP1 |
SNX9 |
β-actin |
B |
AMPH |
CAV1 |
CLTC |
DYNAP |
EPN2 |
KCNJ5 |
MYO5B |
PLD2 |
RAB22A |
RBSN |
SPAG9 |
GAPDH |
C |
AP2A1 |
CAV2 |
CTTN |
ECT2 |
EPN3 |
KLC1 |
MYO6 |
PRKCI |
RAB35 |
REPS2 |
SRC |
LDHA |
D |
AP2B1 |
CAV3 |
DCTN1 |
EEA1 |
EPS15 |
KLC2 |
MYOF |
PTRF |
RAB4A |
RHOA |
STX5 |
NONO |
E |
AP2S1 |
CDC42 |
DCTN2 |
EHD1 |
EXOC5 |
KRAS |
PAK1 |
RAB10 |
RAB5A |
ROCK1 |
SYNJ1 |
PPIH |
F |
ARF1 |
CEP164 |
DENND1A |
EHD2 |
FLOT1 |
LAMP1 |
PAK2 |
RAB11A |
RAB7A |
SDCBP |
UBB |
GDC |
G |
ARF6 |
CLINT1 |
DNAL1 |
EHD3 |
FLOT2 |
MAPK1 |
PARD3 |
RAB11FIP2 |
RAB8A |
SNX1 |
VPS45 |
PPC |
H |
ARHGAP10 |
CLTA |
DNM1 |
EHD4 |
HDAC6 |
MAPK8IP2 |
PARD6A |
RAB11FIP3 |
RACGAP1 |
SNX4 |
WWCI |
NTC |
* gene selection may be updated based on new research and development
Catalog No. | GK022 |
---|---|
Country of Manufacture | United States |
Product Code | GQH-ECY |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | GQH-ECY is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |
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