分子量:36.7 kDa (pro-form) and 29 kDa (mature-form)

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Cathepsin B, His Tag by the LAL method.

Buffer:50mM Tris-HCl, 150mM NaCl, pH 8.0

生物活性:Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100μl reaction mixture containing 25 mM MES, pH 5.0, 0.01 g rhCathepsin B, 10 M reaction substrate. The specific activity is >2800 pmol/min/g.

产品特性:rh CTSB is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 36.7 kDa (pro-form) and 29 kDa (mature-form). The predicted N-terminus is Arg18 (pro-form) or Phe74 (mature-form). Due to glycosylation, The reducing (R) protein migrates as 43 kDa (pro-form) band  in SDS-PAGE and there may be a 34 kDa (mature-form) band.

产品背景:Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.